User guides

Choose the task you want to complete and follow the illustrated steps or video.

Getting started and projects

1.1Back up and reopen a project1.2Adjust interface settings

Import and review originals

2.1Import images and multipage TIFFs2.2Set companions and remember the format2.3Assign companion images manually2.4Review companion images

Edit and label strips

3.1Crop and save a band3.2Use consistent crop dimensions3.3Adjust and reset image display3.4Position crops with Merge3.5Add molecular-weight labels3.6Save proteins and molecular weights3.7Compare originals for a protein

Figure layout and export

4.1Build a figure4.2Adjust text, rows and strip dimensions4.3Create + / − condition rows and group labels4.4Add borders to strips4.5Copy a figure layout4.6Copy rows, text and formatting4.7Review source-image reuse4.8Export a Figure

Densitometry

5.1Quick intensity analysis5.2Analyze Figure strip intensity

Biological replicates and statistics

6.1Bind biological replicates6.2Calculate and review replicate results6.3Choose the design and run statistics

Antibodies and usage tables

7.1Build and import an antibody library7.2Record antibody use on a strip7.3Export an antibody table for a figure

Source-data reports

8.1Export Figure source data8.2Export biological replicate source data