Caspase-1 22915-1-AP
Proteintech · WB · 1:3000 · 4–20% gradient gel
The early blots were clear. This time, p10 appears with a longer exposure, while other cleavage bands show little difference.
Pyroptosis-related antibody WB notes
This topic groups existing records relevant to pyroptosis research. Signal changes alone do not establish pyroptosis. Different fragments and stimulation contexts require separate interpretation.
Read the catalog-specific records
Caspase-1 p20 A23429
ABclonal · WB · 1:3000 · 4–20% gradient gel
After about three years, p20 is still easy to detect; the inhibitor groups also show an interesting intermediate-sized band.
GSDMD NT A24059
ABclonal · WB · 1:3000 · 4–20% gradient gel
The p30 NT band is prominent, and its reduction after inhibitor treatment is easy to see.
GSDME A28230
ABclonal · WB · 1:3000 · 4–20% gradient gel
The full-length band drops markedly while p35 NT is clear, making the processing pattern easy to see.
GSDME NT A26197
ABclonal · WB · 1:3000 · 4–20% gradient gel
I have had good results with both catalog versions; this test shows clear changes at p35 and approximately 13 kDa.
GSDMD #39754
CST · Cell Signaling Technology · WB · 1:3000 · 4–20% gradient gel
A favorite after about three years of use, with clear p30 and p43 processing patterns.
The approximately 13 kDa GSDME NT band and 30–40 kDa Caspase-1 p20 signals remain unidentified. Molecular weight alone is insufficient to name these bands.