cIAP1 A19688
ABclonal · WB · 1:3000 · 4–12% gel
After more than a year of use, the background remains clean, with cIAP1 loss visible in several cell-death models.
Apoptosis-related antibody WB notes
Find records for initiator and effector caspases, BCL2-family and IAP proteins. Different products, gels, blocking and exposure conditions do not form a matched performance ranking.
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XIAP A20846
ABclonal · WB · 1:3000 · 4–12% gel
The background is fairly clean; full-length XIAP decreases while an approximately 30 kDa band becomes stronger.
Caspase-3 AF5132
Beyotime · WB · 1:3000 · 4–12% / 4–20% gradient gels
Target bands and changes are visible despite numerous extra bands; p17 is less prominent at 120 min than at 60 min.
Cleaved caspase-3 p12 A28349
ABclonal · WB · 1:3000 · 4–20% gradient gel
p12 appears at the expected position, increases clearly after stimulation and decreases or disappears with inhibitors.
Caspase-7 27155-1-AP
Proteintech · WB · 1:3000 · 4–12% / 4–20% gradient gels
Recent runs have more extra bands, but full-length loss and changes in the smaller bands are still visible.
Caspase-9 A18676
ABclonal · WB · 1:3000 · 4–12% gradient gel
Full-length protein and p10 can be detected together; the faint p10 signal is clearer with a longer exposure.
BAK: Two-antibody comparison AF1591 / A23523
Beyotime / ABclonal · WB · 1:3000 · 4–12% gel
Both antibodies give clear target bands and clean backgrounds; total BAK does not noticeably increase within 2 h.
BCL2 AG1225
Beyotime · WB · 1:3000 · 4–12% gel
After about three years of use, target bands remain clear and the background clean; BCL2 is fairly stable in this experiment.
PUMA AF1204
Beyotime · WB · 1:3000 · 4–12% gel
After about three years of use, the background remains clean; PUMA decreases over this stimulation time course.
A lower full-length band, cleavage change or clean background alone does not establish specificity. The BAK record contains two antibodies; match catalog numbers to figure labels.