1
00:00:01,204 --> 00:00:09,355
Drag the folder containing all eight original images into the image workspace.

2
00:00:09,355 --> 00:00:17,889
This prompt asks whether to find companion images. Click
Cancel first, then set the rules using the imported images.

3
00:00:17,889 --> 00:00:27,300
All eight originals are now in the library. They cover four image
types for reviewing your results and preparing source data.

4
00:00:27,300 --> 00:00:36,602
Chemi is the chemiluminescence image. It records
the protein signal; the horizontal signals are bands.

5
00:00:36,602 --> 00:00:44,711
White is the white-light image. The membrane
and marker on the left help you locate the bands.

6
00:00:44,711 --> 00:00:54,188
Merge is the overlay image. It combines the chemiluminescence
and white-light images so you can compare their positions.

7
00:00:54,188 --> 00:01:03,992
Ponceau is the Ponceau S staining image. It shows protein staining across
the membrane, and is called Whole-membrane photo in the software.

8
00:01:03,992 --> 00:01:13,584
This is one membrane cut into two halves. The upper half,
1-1, detects HSP90; the lower half, 1-2, detects GSDMD.

9
00:01:13,584 --> 00:01:22,847
In the densitometry tutorial, we will use HSP90 as the loading control
for GSDMD. Both halves share the whole-membrane photo.

10
00:01:22,847 --> 00:01:32,448
Four image types from the same membrane section, protein and
exposure form a matched image set. Here, two sets contain eight images.

11
00:01:32,448 --> 00:01:39,141
We recommend naming files in this order: date, membrane ID, section ID,
protein name, and image type.

12
00:01:39,141 --> 00:01:48,789
For 20261006 1-1 HSP90, 20261006 is the
date. The first 1 identifies the membrane.

13
00:01:48,789 --> 00:01:57,442
The 1 after the hyphen identifies the section. For the
lower half, use 1-2 and replace HSP90 with GSDMD.

14
00:01:57,442 --> 00:02:05,750
The shared beginning is the filename body. Add Chemi,
White, Merge or Ponceau at the end to identify the image type.

15
00:02:05,750 --> 00:02:12,198
With the matching rules set, filenames like these let the software recognize
and match all four images automatically.

16
00:02:12,198 --> 00:02:19,943
Other filenames are supported too. See Troubleshooting Image Recognition
and Matching for how to handle them.

17
00:02:19,943 --> 00:02:25,036
Select the imported source folder, then open Image matching settings.

18
00:02:25,036 --> 00:02:30,322
Use automatic detection and keep
Automatically link related images by filename enabled.

19
00:02:30,322 --> 00:02:39,562
Click Try my filenames. Move the panel aside so you
can see the library and the example rows together.

20
00:02:39,562 --> 00:02:48,304
Drag the HSP90 chemiluminescence image from the upper
half into the Chemiluminescence image example row.

21
00:02:48,304 --> 00:02:57,303
Drag its white-light image, overlay image and
whole-membrane photo into their corresponding rows.

22
00:02:57,303 --> 00:03:05,333
The four filenames are filled in automatically. Their types
and filename body match, so there is no need to type them.

23
00:03:05,583 --> 00:03:13,639
Here is another example. The image types are recognized, but different
filename bodies leave the companion images unmatched.

24
00:03:13,639 --> 00:03:18,335
See Troubleshooting Image Recognition and Matching for the fixes.

25
00:03:18,335 --> 00:03:23,975
After checking the examples, click Save settings
to apply the matching rules to this folder.

26
00:03:23,975 --> 00:03:32,070
The eight images now form two matched sets, HSP90 and
GSDMD, based on their filename bodies and image types.

27
00:03:32,070 --> 00:03:41,817
Next, right-click the GSDMD chemiluminescence image
from the lower half, 1-2, and choose View linked images.

28
00:03:41,817 --> 00:03:59,549
Enlarge each of the four originals. Check that the
complete filenames and image contents correspond.

29
00:03:59,549 --> 00:04:05,468
Once all four are correct, click Save and confirm companion images.

30
00:04:05,468 --> 00:04:26,416
Now open View linked images for HSP90 from the
upper half, 1-1. Enlarge and check all four originals.

31
00:04:26,416 --> 00:04:34,037
The four HSP90 images also match. Click
Save and confirm companion images.

32
00:04:34,037 --> 00:04:42,375
Both matched image sets have been checked and saved
separately. The library still contains the same eight originals.

33
00:04:42,375 --> 00:04:54,376
You can also use Import folder or Import WB images, or select
several files and drag them in. Choose whichever suits your files.

34
00:04:54,376 --> 00:05:04,708
Finally, click Back up project to save the original images and
their matching relationships. Use this project in the next tutorial.

35
00:05:04,708 --> 00:05:09,956
Imported images are copied to the local data folder you selected for this
workspace.

36
00:05:09,956 --> 00:05:16,788
Back up project packages the originals, their matching links and saved edits
into one .wbfig file.

37
00:05:16,788 --> 00:05:24,125
Copy the backup to another computer, then use Open project in WB Figure
Studio to continue working.