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Click Open project and open the saved Figure project.

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This Figure already contains GSDMD and HSP90. Both
matched image sets have been checked and saved.

3
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Source data are the full acquired images. The bands in
the Figure come from crop regions within those images.

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Click Source data.

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The left panel lists the two image sets used in this
Figure. The right panel previews the export page.

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Select GSDMD to view the four source
images for the lower half of the membrane.

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The filenames, image types, and confirmed
status all belong to this GSDMD image set.

8
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Select HSP90 to view the four source
images for the upper half of the membrane.

9
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Both sets are confirmed, so the software uses their saved image associations.

10
00:00:57,730 --> 00:01:02,875
Expand Export settings to choose the image columns to include.

11
00:01:02,875 --> 00:01:09,515
Keep the chemiluminescence image, white-light
image, overlay image, and whole-membrane photo.

12
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Below, select both the GSDMD and HSP90 image sets.

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Both sets use the same whole-membrane photo.
It can be included with each protein in the export.

14
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Drag the handle beside the white-light column to move
it to the first column, so the marker is easy to check.

15
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Rename this column White light and marker.

16
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Press Enter to save the column heading.

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Choose English as the export language. Custom
column headings keep the text you entered.

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Collapse Export settings to view the updated page.

19
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White light is now the first column. GSDMD and HSP90 each have
their own row, and every image keeps its original proportions.

20
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Before exporting, check that both image sets are
complete and the column headings are correct.

21
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Click Export PDF to save this source-data page as a PDF file.

22
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Open the saved PDF and view both full image sets in the selected column order.

23
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This is the actual exported page. You can see
GSDMD, HSP90, and all four column headings.

24
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Zoom in to check the image boundaries and protein names.

25
00:02:32,848 --> 00:02:38,309
Return to the full-page view and check that all the content is included.

26
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Return to the software to save a file you can edit in PowerPoint.

27
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Click Export PPTX.

28
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Open the saved PPTX and check the two
image sets, column headings, and page layout.

29
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The upper-half and lower-half images keep their own
proportions, and the whole-membrane photo is shown in full.

30
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Return to the software, keeping these export settings.

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Click Back up project to save the source
images, strips, Figures, and export settings.

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Reopen this project next time to continue organizing source data from this point.