1
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Each of these three examples uses a separate project.
First, import the set with an incorrect image-type suffix.

2
00:00:10,853 --> 00:00:18,874
This is an HSP90 overlay image, but its Chemi suffix
caused it to be detected as a chemiluminescence image.

3
00:00:18,874 --> 00:00:22,443
Open View linked images.

4
00:00:22,443 --> 00:00:29,443
Expand Image types and experiment details, then
click Edit image types or experiment details.

5
00:00:29,443 --> 00:00:33,369
Change Image type to Overlay image.

6
00:00:33,369 --> 00:00:39,941
Check the filename and image type, then click Save links.

7
00:00:39,941 --> 00:00:45,976
The overlay is now in the correct slot, and
all four companion images are present.

8
00:00:45,976 --> 00:00:51,680
After checking the set, click Save and confirm companion images.

9
00:00:51,680 --> 00:00:55,583
Back up this repaired project.

10
00:00:55,592 --> 00:01:05,807
In the second project, all four image types are imported,
but different filename bodies prevent automatic matching.

11
00:01:05,807 --> 00:01:14,020
Open the GSDMD chemiluminescence image. Its
other companion images have not been matched yet.

12
00:01:14,020 --> 00:01:20,889
Return to the library and filter for these four GSDMD images.

13
00:01:20,889 --> 00:01:24,054
Click Select all.

14
00:01:24,054 --> 00:01:27,917
Then click Link companion images.

15
00:01:27,917 --> 00:01:34,766
Check each filename and image type: chemiluminescence,
white-light, overlay and whole-membrane photo.

16
00:01:34,766 --> 00:01:42,846
After confirming that these four images belong to one set, click Save links.

17
00:01:42,846 --> 00:01:49,426
Open the companion images again. All four now form a complete set.

18
00:01:49,426 --> 00:01:55,263
After checking the set, click Save and confirm companion images.

19
00:01:55,263 --> 00:02:00,500
Back up the project to preserve these matching relationships.

20
00:02:00,510 --> 00:02:09,731
The third project uses camera filenames. They indicate
neither the image types nor a shared filename body.

21
00:02:09,731 --> 00:02:15,829
Click Assign image types manually, and
classify the files by their image content.

22
00:02:15,829 --> 00:02:21,208
Drag the chemiluminescence original into Chemiluminescence image.

23
00:02:21,208 --> 00:02:25,667
Drag the white-light original into White-light image.

24
00:02:25,667 --> 00:02:30,182
Drag the overlay original into Overlay image.

25
00:02:30,182 --> 00:02:34,737
Drag the whole-membrane photo into its matching slot.

26
00:02:34,737 --> 00:02:39,606
Check the four types, then click Save image types.

27
00:02:39,606 --> 00:02:43,576
Filter for these four images again.

28
00:02:43,576 --> 00:02:46,658
Click Select all.

29
00:02:46,658 --> 00:02:50,397
Open Link companion images.

30
00:02:50,397 --> 00:02:56,620
Check the four filenames and image types, then save the association.

31
00:02:56,620 --> 00:03:02,762
To organize the set by experiment, open Image types and experiment details.

32
00:03:02,762 --> 00:03:09,932
Experiment details, which are optional, organize
this set by experiment, membrane and protein.

33
00:03:09,932 --> 00:03:16,347
Enter 20261006 WB Example as the experiment name.

34
00:03:16,347 --> 00:03:20,126
Enter 1 as the membrane ID.

35
00:03:20,126 --> 00:03:26,257
Enter 1-2 as the membrane section ID, corresponding to the lower half.

36
00:03:26,257 --> 00:03:30,681
Enter GSDMD as the protein name.

37
00:03:30,681 --> 00:03:35,219
Choose Target protein as the protein category.

38
00:03:35,219 --> 00:03:42,132
Check these details, then click Apply to these companion images.

39
00:03:42,132 --> 00:03:49,686
Open the set again. All four images and the
experiment details are correctly associated.

40
00:03:49,686 --> 00:03:54,368
Click Save and confirm companion images.

41
00:03:54,368 --> 00:04:01,235
Finally, back up the originals, image types,
experiment details and matching relationships.

42
00:04:01,235 --> 00:04:10,584
Use automatic matching for files with consistent names. Use
these manual tools when a set needs fixing or additional details.